Colon Cell Lines

NCM460 Cell Line
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Description, Maintenance, and Harvest

General Description: The NCM460 cell line is an epithelial cell line derived from the normal colon of a 68-year-old Hispanic male (*Moyer et al.,1996). The line was selected for in vitro growth. It was not infected or transfected with any exogenous genetic information. The cells express cytokeratins, villin, and other colonic epithelial cell antigens, but are negative for antigens associated with other cell types. Some of the cells are positive for mucin synthesis. They do not grow in soft agar and are non-tumorigenic. They have fastidious growth requirements and must be maintained in INCELL’s enriched M3:10TM medium for long-term in vitro
culture with maintenance of an appropriate phenotype.

NCM356 Cell Line

Description, Maintenance, and Harvest

General Description:  The CD-NCM356 cell line is an epithelial cell line derived from the normal colon mucosa wide margin resection of a 65-year-old black male with rectal adenocarcinoma (Stauffer et al., 1995). The cells express cytokeratins, villin and other colonic epithelial cell antigens, but are negative for antigens associated with other cell types. Some of the cells are positive for mucin synthesis and a subset of “tumor-associated” antigens (e.g. mutantp53, TAG72). They do not grow
in soft agar and are non-tumorigenic. They have fastidious growth requirements and must be maintained in INCELL’s enriched M3:10TM medium for long-term in vitro culture with maintenance of an appropriate phenotype.

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