Table 1 shows media designations and supplement options. M3™ Base Medium is a proprietary formula that is a serum-free, highly enriched liquid culture medium for human stem cells and other progenitor and fastidious cell types. It is also useful for culture of animal-derived stem and progenitor cells, tumor cells, and tissues. It is the M3™ Base Medium to which serum, plasma, or other factors may be added to meet the needs of the individual cell-type. The M3™ base medium is used to make the M3™ family of serum-supplemented cell culture media. This includes M3:10™ which is the same as M3™ Base Medium supplemented with 10% v/v fetal bovine serum (FBS). M3™ Base is provided to international or other clients who need or want control over their sources of serum, and as the base medium to optimize FBS and other supplements for specific cell types or tissues (Table 2).
The extended M3™ media family includes M3:BaseA™, M3:BaseAC™, M3:BaseF™, M3:BaseFC™, M3:10A™ and M3:10AC™. Media suffix designations of “F”, “A” and “C” refer, respectively, to indicate Free of antibiotics, Antibiotic mixture (Gentamicin, Clindamycin and Amphotericin) and Clear (no phenol red). M3D™ (M3DEF) is the chemically defined solution of salts, sugars, amino acids, and buffers to which the growth supplements are added to make M3™ Base media. M3™ media are generally available in 100 mL and 500 mL volume sizes, but custom orders can be made for other sizes. SMX™ is available in a 25 mL volume size. M3:D™ (M3DEF) and the same formula without phenol red, i.e., M3:DC™ (M3DEC), are the chemically defined platform solution of salts, sugars, amino acids, and buffers to which the growth factors and other supplements are added to make the M3™ Base medium formula. M3:D™ or M3:DC™ are commonly used as a “control”, “holding” or “shift-down” media in studies where M3™ media are being used for growth or in vitro testing, and a control group requires absence of growth stimulating factors.